| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,964,457 | 0 | T | G | 52.9% | 18.3 / 20.1 | 17 | V322G (GTG→GGG) | wecB | UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964369‑3964545 | aTTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTg < 2:284554/90‑1 (MQ=255) aaCCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTGGTAGGGACGGGGAAACAGCGaa > 2:207674/1‑90 (MQ=255) aCCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGTGGGGCCTGGAGGGACGGGTAAGCAGCGagc > 1:195898/1‑88 (MQ=255) ccTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAAtt < 1:375901/90‑1 (MQ=255) gtgCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGGAGGGACGGAAAAGCAGCGGAATGtt > 1:694721/1‑89 (MQ=255) gTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTGGTAGGCGCGGGTAAGCAGCGAAATGTCGAGGaa > 1:369013/1‑90 (MQ=255) tGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTaaa < 2:694721/90‑1 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGTCTTGGAGGGACGGGTAAGCAGGGAATTTTTGGGGGAGTGGCGCGGCTTTTtaaa > 1:350327/1‑90 (MQ=255) gCGTCCGGAAGCGGTGACGGCGGGGAAGGGGCGGCTGGGAGGCACGGAAAAGCAGCGAAATGTCGAGGGAGGGAAGGGGCTTTTtaaaaa > 1:375060/1‑88 (MQ=255) tCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGCACGGGTAAACAGCGAAATGTTGGGGGAGGGACGCGGCTTTTTAAAAACGa > 1:533764/1‑90 (MQ=255) ccGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGaa < 2:500571/90‑1 (MQ=255) ccGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGGGGGACGGGTAAGCAGGGAAATGTGGAGGGAGTGGCGCGGCTTTTTAAAAAAGaa > 1:379643/1‑90 (MQ=255) ggTGACGGCGGGTACGGGGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGGAGGGACGCGGCTTTTAAAAGACGAAAACGaaaa > 1:694298/1‑88 (MQ=255) gACGGCGGGTACGGGGGGGCTGGGAGGGACGGGTAAAGAGCGAATTTTCGGGGGAGGGGGGGGGCTTTTTAAAAAAGAAAAAGAaaatta > 2:176071/1‑88 (MQ=255) gggTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTAt < 2:195898/90‑1 (MQ=255) gTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCcgc < 2:190134/90‑1 (MQ=255) gTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCcgc < 1:176071/90‑1 (MQ=255) | ATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964369‑3964545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |