| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 55.6% | 9.5 / 20.2 | 18 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (10/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG > NZ_CP009273/535930‑536099 | cGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGTTAAGGTGTGtt < 2:235032/90‑1 (MQ=255) tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGGTAAGGGGGGGTTTTGTTTTAtttttc > 2:141222/1‑88 (MQ=255) tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGGTAAGGGGGGGTTATGTTCAATTTTGCAGTCAGCggc > 2:579354/1‑90 (MQ=255) gcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaa < 1:558705/90‑1 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCT‑CCGGTTAAGGGGTGGTTTTGTTTAATTTTTGAGGCAGGCGCGGAAGGCTGtttt > 2:147054/1‑88 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCT‑CCGGTTAAGGGGGGGTTTTTTTTAATTTTTCGGTCAGCCGCGGAAACCCGtttt > 2:384059/1‑88 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGTTAAGGGGGGTTTTTTTTCAATTTTTCAGTCAGCCCCGAAAACCTGTTAt > 2:280380/1‑90 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCCGGTAAGGGGGGGTTTTTTTTCAATTTT‑CAGGCAGCCGCCGAAGACTGTTTTTAggg > 2:313609/1‑89 (MQ=255) aTGCCCGTCCTTGCGGCGGGCATTCT‑CCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGGCAGCCGCGAAAGCCTGTTTTCAGGAtttta > 1:570338/1‑88 (MQ=255) gCCCGTCCTTGCGGCGGGGATTCT‑CCGGCTTAGGGGGGGTTGTGTTCAATTTTCCCGTCAGCCGCGGAAGGCTGTTATCAGGAattaatc > 1:703017/1‑86 (MQ=255) gTCCTTGCGGCGGGCATTCT‑CCGGGTAAGGGGTGGTTTTTTTCAATTTTTCAGTCAGCCGCGAAAACCTGTTTTTAGGATTTTAGTgggt > 2:291786/1‑88 (MQ=255) cggGCATTCT‑CCGGGTAAGGGGTGTTTATGTTCAATTTTTCAGGCAGCCGCGAAAGCCTGTTATCAGGGTTTTAGTGGGTtttttttttt > 2:236591/1‑90 (MQ=255) gggCATTCT‑CCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTAttt < 1:291786/90‑1 (MQ=255) ccGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAaca < 2:433699/90‑1 (MQ=255) ggTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac > 1:481816/1‑89 (MQ=255) ggTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac < 2:481816/89‑1 (MQ=255) ggTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACg > 1:477695/1‑90 (MQ=255) aaGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTtg < 1:236591/90‑1 (MQ=255) | CGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCT‑CCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG > NZ_CP009273/535930‑536099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |