Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,613,5550TG40.0% ‑2.6 / 10.5 10L60R (CTG→CGG) marRmultiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base G (2/2);  total (6/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTT  >  NZ_CP009273/1613485‑1613636
                                                                      |                                                                                 
gATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtc                                                                <  1:328116/90‑1 (MQ=255)
         gcgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGa                                                       >  1:24976/1‑90 (MQ=255)
           ggCACAGTTTAAGGTGCTCTGCTCTATACGCTGCGCGGCGTGTATTACTCCGGTTGAACGGAAAAAGGTATTGTCGGTCGACCTGGGAGc                                                     >  2:90735/1‑90 (MQ=255)
             cacaGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACGGAAAAAGGTATTGTCGGTCGACCTGGGAGCAc                                                   <  2:83762/90‑1 (MQ=255)
             cacaGTTTAAGGTGCTCTGCTCTATACGCTGCGCGGCGTGTATTACTCCGGTTGAACGGAAAAAGGTATTGTCGGTCGACCTGGGAGCAc                                                   <  1:90735/90‑1 (MQ=255)
                                         cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtc                                                                >  1:153270/1‑49 (MQ=255)
                                         cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTgtcggtc                                                                <  2:153270/49‑1 (MQ=255)
                                                            ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCCGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAgg    >  2:263646/1‑90 (MQ=255)
                                                              ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt  >  1:23780/1‑90 (MQ=255)
                                                              ggTTGAACGGAACAAGGTATTGTCGGTCGACCTGGGGGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt  >  2:206692/1‑90 (MQ=255)
                                                                      |                                                                                 
GATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTT  >  NZ_CP009273/1613485‑1613636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑