| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,254,480 | 0 | A | C | 53.8% | 9.2 / 13.6 | 13 | V137G (GTG→GGG) | fruA | PTS fructose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCC > NZ_CP009273/2254400‑2254547 | caATCCCCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCACCCACCAGc < 1:5639/90‑1 (MQ=255) caTCCCCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTCCATTCCCCGCGCCAACAGAACCACGGGTTTAAACT‑TTCACCCACCAGcc < 1:227012/89‑1 (MQ=255) gctgctGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCACCCACCAGCCACGTTTTTTCGCt > 2:23630/1‑90 (MQ=255) gtgtTTGCTTTCCCCGCGCCAACAGAACCCCGGTTTTCAACT‑TCCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 1:23630/90‑1 (MQ=255) gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 2:172418/90‑1 (MQ=255) gTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc < 2:107676/90‑1 (MQ=255) gATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc > 1:15406/1‑90 (MQ=255) cATTACCCGCGCCAACAGACCCACGGGTTTCAACT‑TTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATaa < 2:65155/90‑1 (MQ=255) cccGCGCCAACAGACCCAGGGGTTTCAACT‑TTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtg < 2:298858/90‑1 (MQ=255) ccGCGCCACCAG‑ACCCCGGGTTTTCACTCCCCCCCCCCCAGCCACTTTTTTTCCCTTCGTTTTCAATGGCTTCAGCCGCCATAAAGgtgt < 1:250305/90‑1 (MQ=255) aaaaCCAGGGGTTTCAACT‑TTCCCCCACCAGCCACGTTTTTTCGCTTCGTTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGcc < 2:166388/88‑1 (MQ=255) tCAACT‑TTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtgtgt > 1:171737/1‑69 (MQ=255) tCAACT‑TTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtgtgt < 2:171737/69‑1 (MQ=255) | CAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACT‑TTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCC > NZ_CP009273/2254400‑2254547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |