| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,659,062 | 0 | C | A | 53.8% | 3.1 / 15.4 | 13 | A37S (GCG→TCG) | gadX | acid resistance transcriptional activator GadX |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base A (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATA > NZ_CP009273/3658975‑3659137 | gCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCaa > 2:276098/1‑90 (MQ=255) cccTTGACACGAATAAAAAATTTTCAACCCACTTATCTACTCGATTTTGGC‑TTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGc < 2:111040/90‑1 (MQ=255) cTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGa < 2:81900/90‑1 (MQ=255) cacaAATAAAAAATTTTCAACCCCTTTTTCTCCTCAATTTGGGC‑TTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc < 1:104043/90‑1 (MQ=255) cacGAAAAAAAAATTTTCAACACACTTATCTACTCGATTTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTc < 2:11609/90‑1 (MQ=255) caaaaaTAAAAAATTTCAAACCCCCTTACCTACTCGATTTGGGC‑TTCCACCCCAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTc < 2:274344/86‑1 (MQ=255) acGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCa > 1:274344/1‑90 (MQ=255) cAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTaa > 1:319837/1‑90 (MQ=255) atttttCAACACATTTATCTACTCGATTTTGGC‑TTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCa < 2:178086/88‑1 (MQ=255) tCAACCCCCTTATTTACT‑AAATTTGGCTTTCCACCCAAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 1:195777/90‑1 (MQ=255) ccAACACCCTTATCTACTCAATTTTGTC‑TTCCACCCAAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 1:206825/89‑1 (MQ=255) acatTTATCTACTCTATTTTGTC‑TTCCCCCCAAAAAAACCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat < 1:276098/90‑1 (MQ=255) ttGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa > 1:31552/1‑90 (MQ=255) | GCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATA > NZ_CP009273/3658975‑3659137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |