| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,774,215 | 0 | T | G | 57.1% | 7.5 / 11.7 | 14 | G343G (GGT→GGG) | lldD | quinone‑dependent L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATC > NZ_CP009273/3774135‑3774287 | cGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAc < 1:183658/90‑1 (MQ=255) gTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAAcc < 1:234097/90‑1 (MQ=255) gcgcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTGGCTAACCTGCTAAAt > 2:197894/1‑90 (MQ=255) gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAAACTGCTAAATCt > 2:268127/1‑90 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGaaaa < 2:120992/84‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGaaaa > 1:120992/1‑84 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGGGGCGa > 2:262949/1‑90 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGa > 1:14388/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGa > 2:91784/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGa > 1:131767/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGGGGCGATgg > 1:270489/1‑89 (MQ=255) gCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCCGCTAAATCTGGTCGAAAAAGAGATGAAAGGGGCGAGGACg > 1:161758/1‑90 (MQ=255) ggCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATc < 1:128156/79‑1 (MQ=255) ggCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATc > 2:128156/1‑79 (MQ=255) | CGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATC > NZ_CP009273/3774135‑3774287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |