Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273117,2520TG53.8% 1.8 / 11.8 13T263P (ACC→CCC) aroParomatic amino acid transporter AroP
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (7/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAAC  >  NZ_CP009273/117167‑117318
                                                                                     |                                                                  
aCGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCAc                                                                <  1:263062/90‑1 (MQ=255)
aCGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCAc                                                                >  1:283426/1‑90 (MQ=255)
 cGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCACg                                                               >  2:276230/1‑90 (MQ=255)
     gTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCAt                                                           <  1:16837/90‑1 (MQ=255)
     gTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAAGCGGGGCCCCCGCAt                                                           >  2:241270/1‑90 (MQ=255)
     gTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGGAACGCGGGGCCACGGCAt                                                           >  1:41101/1‑90 (MQ=255)
               ttCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGc                                                 >  1:264268/1‑90 (MQ=255)
               ttCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCACGGCATCAGGGAGAGc                                                 >  1:218692/1‑90 (MQ=255)
                      caAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACgg                                          <  2:208217/90‑1 (MQ=255)
                                             aaGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATc                   <  2:179902/90‑1 (MQ=255)
                                                    gCACAAACGGACTGGTATCGGCGGTAACGCGGGGCCCCCGCATCAGAGAGAGGAGAACGGCCAACGAACCAAAATAGAAAAACAGGaggg            >  1:312497/1‑87 (MQ=255)
                                                        aaaCGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTa        <  2:235638/90‑1 (MQ=255)
                                                              aCTGGTATCGGCGGTAACGCGGGGCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAAAATAGAAAAACAGGAAGCGGGAGATAAc  >  1:68515/1‑90 (MQ=255)
                                                                                     |                                                                  
ACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCGGGTCCACGGCATCAGTGAGAGCAGAACGGCTAACGAACCAATATAGAAAATCAGGATGCGGTAGATAAC  >  NZ_CP009273/117167‑117318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑