Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,765	0	C	T	100.0%	26.4 / NA	9	intergenic (+134/‑58)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/3);  total (6/3)

CGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGG  >  NZ_CP009273/49694‑49841                                                                        |                                                                             cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                            <  2:268481/90‑1 (MQ=255)             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                                >  1:83312/1‑90 (MQ=255)              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                         <  1:235675/64‑1 (MQ=255)              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                         >  2:235675/1‑64 (MQ=255)              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCg                                               >  1:296726/1‑90 (MQ=255)              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCg                                               >  2:271077/1‑90 (MQ=255)                   gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt                                          >  1:21013/1‑90 (MQ=255)                                              ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc               >  1:146894/1‑90 (MQ=255)                                              ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTATCGGGAAATCTCAATGATCa              >  1:308257/1‑90 (MQ=255)                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa              <  1:222989/90‑1 (MQ=255)                                                           tcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg  <  2:219375/90‑1 (MQ=255)                                                                        |                                                                             CGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGG  >  NZ_CP009273/49694‑49841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑