| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,155,588 | 0 | A | G | 54.5% | 6.1 / 12.0 | 11 | D215G (GAC→GGC) | mdtD | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATC > NZ_CP009273/2155500‑2155668 | ccGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGAc < 2:337715/90‑1 (MQ=255) gAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGGATTTACCCCGGGGCCGGGCgg > 1:187090/1‑90 (MQ=255) aTGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCATGGGGGGTTTTAACCC‑GGCGCTGGGCGGGCGGAAAggg > 1:303754/1‑89 (MQ=255) aTGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGGATTAACCCTGGCGCTGGGCGGCAGTAAAggg > 2:271985/1‑89 (MQ=255) aTGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGGATTAACCCTGGCGCTGGGCGGCAGTAAAggg > 2:96065/1‑89 (MQ=255) ctctcCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACg < 2:303754/90‑1 (MQ=255) cGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGc < 2:233664/90‑1 (MQ=255) tttATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCt < 2:477980/90‑1 (MQ=255) ttATTGCTGGCGGTTGGCAT‑GGCGGTATTAAACCCGGCGCTGGGCGGCAGTAAAGGGACAGGGTTTTCGCCGCCGACGGTTGCAGGcccg > 1:169559/1‑88 (MQ=255) tGGCGGTTGGCAT‑GGCGGGATTTACCCCGGGGCTGGGCGGGAGTAAAAGGATAGGGTTTTTCCCGCCGACGGTTTCCGGCCTGGgcgccg > 1:521592/1‑88 (MQ=255) tGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATc > 1:404288/1‑90 (MQ=255) | CCGAACTACACCATGCAGACGCGGCGCTTTGATCTCTCCGGATTTTTATTGCTGGCGGTTGGCAT‑GGCGGTATTAACCCTGGCGCTGGACGGCAGTAAAGGTACAGGTTTATCGCCGCTGACGATTGCAGGCCTGGTCGCAGTTGGCGTGGTGGCACTGGTGCTTTATC > NZ_CP009273/2155500‑2155668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |