| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,678,659 | 0 | A | C | 60.0% | 12.7 / 15.0 | 15 | Y70D (TAT→GAT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/9); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGC > NZ_CP009273/2678574‑2678748 | ccGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCg > 2:415452/1‑90 (MQ=255) ggATGTTGGCGTAGCCAGCGCCGAACTTTTTTTCCCCACGATCGATCGCCGGTTTTTCAACAATATCAACATCCCCGCAACCGCCgtagt < 1:22691/90‑1 (MQ=255) aCGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGtagtag > 1:39422/1‑90 (MQ=255) gcgcCGAACAGTTCTTTCGCACGATCGATCGCCAGTTATTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATaa > 1:288506/1‑90 (MQ=255) cGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCtt > 1:54488/1‑90 (MQ=255) aaaCATTTCTTCCGCACGATCGACCGCCATTTTTTCAACGATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 2:131628/89‑1 (MQ=255) aaaCAGTTTTTCCGCACAATCGATCGCCAGTTGTTAAACGATATCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTc < 2:25614/89‑1 (MQ=255) aaCGGTTCTTCCGCCCGATCGTTCGCCGGTTGTTCAACGATATAAACATCCCCGAAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCa < 2:68715/90‑1 (MQ=255) cgatcgttCGCCGTTTTTTCAACAATATCAACATCCCCCCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:90346/90‑1 (MQ=255) cgatcgatGGGTAGTTATTCAACTATATAACCATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:304428/90‑1 (MQ=255) cgatcgatCGCCAGTTTTTAAACGATATAAAAATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:41097/90‑1 (MQ=255) cgatcgacCGCGATTTTTAAAAAGATACCAACATCCCCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:415452/90‑1 (MQ=255) aGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAAccc > 2:361198/1‑90 (MQ=255) taaaCGATATCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGcc < 2:39422/88‑1 (MQ=255) cAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGCCAGCTGAGAACCCTGCGCCt > 1:418641/1‑90 (MQ=255) aCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGc > 2:244729/1‑90 (MQ=255) | CCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGC > NZ_CP009273/2678574‑2678748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |