| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,794,496 | 0 | T | G | 56.5% | 22.5 / 25.1 | 23 | V58G (GTG→GGG) | nrdI | class Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base G (13/0); total (17/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGG > NZ_CP009273/2794409‑2794583 | cGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGc > 2:223746/1‑90 (MQ=255) cTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCa > 2:177031/1‑90 (MQ=255) aTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGAc > 2:412716/1‑90 (MQ=255) gCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGCCGGGGCGGGGCCACGACAggg > 1:532387/1‑89 (MQ=255) gAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCGACAGGGAAtt > 2:328001/1‑90 (MQ=255) gAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGGGGGGCCACGACAGGGAAtt > 2:444324/1‑90 (MQ=255) aCGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCg < 1:296988/90‑1 (MQ=255) ggTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGGCCCGGCAGGGAATTCGCTTTTTtaa > 1:112338/1‑90 (MQ=255) aCATCCTGATCGTGCCCTCTTACGGCGGCGGGGGGAAGGCTGGCGCGGTGGCCCCGCAAGGAATTTGCTTTTTAAACGCCCAGGCC‑AAcc > 1:286384/1‑90 (MQ=255) cTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCAC‑AAACGGGCg > 2:155855/1‑90 (MQ=255) tGATCGTGCCCTCTTACGGCGGGGGCGGGACGGCTGGGGCGGGGCCACCCCAGGGTATTCGCTTTTTTAACCACGAGCAC‑AACCGGGCga > 1:274385/1‑89 (MQ=255) tGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAATTTGCTTTTTTAAAGACGAGCAC‑AAACCGGCgg > 1:115873/1‑89 (MQ=255) tGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACCACAGGGAATTCGCTTTTTTAAAGACGAGCACAAACCggggg > 1:53973/1‑88 (MQ=255) gTGCCCTCTTACGGCGGCGGCGGGACGGCTGGGGCGGGGCCACCACCGGGAAATCCCTTTTTTAAAGACGAGCAC‑AACCGGGCGTTGCtt > 1:344529/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGGACGGCTGGGGCGGGGCCACGGCAGGGAATTTGCTTTTTTAAAGACGAGGAC‑AACCGGGGGGTGCTTcg > 1:8409/1‑90 (MQ=255) cccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTcgc < 2:112338/90‑1 (MQ=255) ccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTcgcg < 1:223746/90‑1 (MQ=255) ccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTcgcg > 2:255742/1‑90 (MQ=255) ggcggcggTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTAtt < 1:484398/87‑1 (MQ=255) ggcggcggTACGGCTGGCGCGGGGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTAtt > 2:484398/1‑87 (MQ=255) cGGCTGGCGCGGTGCCACGACAGGTAATCCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATc < 1:223380/90‑1 (MQ=255) ggCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAAc < 1:177247/90‑1 (MQ=255) ggTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTgg > 2:169732/1‑90 (MQ=255) | CGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCAC‑AACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGG > NZ_CP009273/2794409‑2794583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |