| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 56.2% | 9.2 / 19.2 | 16 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATG > NZ_CP009273/3214038‑3214208 | gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATc < 1:52451/90‑1 (MQ=255)gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATc > 2:348016/1‑90 (MQ=255) tAACGAAATCGGTTATAACTTTCCCAGGGAGATGTCCCGCCAGCGCGTCCGGGTGGCGGGAACGCTCAATAACCCA‑AAACCGACCCGCAt < 1:315195/90‑1 (MQ=255) aaaTCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATCCGCATTGAAc > 2:489512/1‑90 (MQ=255) ttggCCAGGGAGTTTTCCCGCCGGCGCGTCGGGGGGGCGGGAACGCTAAAAAACGCA‑AAACCGACCCGAATAGAACCGCcactgacactg < 1:173523/87‑1 (MQ=255) ttcgCCAGGGATATTTCCCCCCCGGGCGTAGGGGGGGCCGGACCGCTAAAAAACACC‑AAAACGCCCCGCATTAAACCGCcactgacactg < 1:333836/87‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATCCGCATTGAACCGCcactgacactga > 1:153548/1‑90 (MQ=255) gCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 2:407343/1‑90 (MQ=255) gatgtGTCCCGCCGGCGCGTA‑GGGGGGGCGGAACCCCTAAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:489512/86‑1 (MQ=255) agaTTTCCCCCCGGCGCGTGGGGGGGGCCGGACCCCTAAAAACCGCA‑AAAAGGCCCCGAATGAACCCGCCACTGACACTGACCATTGAAc < 1:61749/90‑1 (MQ=255) agaTGTCCCCCCGGCGCGTACGGGGGGCGGGAACCCTCAAAACCCCA‑AAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:59294/90‑1 (MQ=255) ccGCCAGCGCGTCCGGGGGGCCGGACCGCTAAATAACCCA‑AAAACGACCCGCTTTGAACCGCCACTGACACTGACCATTGAACAGTGTGa < 2:153548/90‑1 (MQ=255) cAATAACGCC‑AAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCt > 2:47238/1‑90 (MQ=255) cccaaaacCGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTggcggc < 2:331566/86‑1 (MQ=255) caaaaaCGCCCCGAATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 1:166976/88‑1 (MQ=255) aaaaCGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATg > 1:20257/1‑90 (MQ=255) | GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCC‑AAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATG > NZ_CP009273/3214038‑3214208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |