| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,498,440 | 0 | C | T | 60.0% | ‑0.0 / 12.4 | 15 | E314K (GAA→AAA) | yhfS | aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAT > NZ_CP009273/3498353‑3498515 | tCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCat < 2:239554/90‑1 (MQ=255) tGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAct < 1:86966/90‑1 (MQ=255) tGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAct < 2:257249/90‑1 (MQ=255) gAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTCActctc > 1:303711/1‑89 (MQ=255) tCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACTCTGCCCCCCCCCGGGaa > 1:506763/1‑90 (MQ=255) tCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTaa < 1:93948/90‑1 (MQ=255) gtgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTTTACTCTGCACCCCCCCGGGaaagcaagg > 2:344262/1‑90 (MQ=255) tgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTTTATTTTTACTCTTCCCCCCCCCGGGaaaggaaggc > 1:379394/1‑90 (MQ=255) tgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACTCTGCACCCCCCCGGGaaagcaaagc > 2:382613/1‑90 (MQ=255) aTTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTGACTCTGCACCCCCCCGGGAAGGCAAGGCAcc > 2:62735/1‑90 (MQ=255) tCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTTGACTCTGCACCCACCGGGGAAGGCAAGGCACcgc > 2:137346/1‑90 (MQ=255) aCGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCct < 1:137346/90‑1 (MQ=255) tCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACCTTGCACCCCCCCGGGGAGGCAAGGGCACGCGCTTTTTCGCCCCTTc > 1:456047/1‑90 (MQ=255) cGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCa < 2:379394/90‑1 (MQ=255) gagCGGCGGGATTTTATATTTCGACTCTGCACCCCCCGGGGAAGGGAAGGCACCGCGCTTTTTCGCCTCTTCCAGCACTCTGGCGGGaaa > 2:532609/1‑89 (MQ=255) | TCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAT > NZ_CP009273/3498353‑3498515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |