Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,498,4470CT57.1% ‑1.0 / 10.7 14S311S (TCG→TCA) yhfSaminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base T (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAT  >  NZ_CP009273/3498362‑3498515
                                                                                     |                                                                    
tGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAct                                                                  <  1:86966/90‑1 (MQ=255)
tGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGAct                                                                  <  2:257249/90‑1 (MQ=255)
   gAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTCActctc                                                               >  1:303711/1‑89 (MQ=255)
                 tCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACTCTGCCCCCCCCCGGGaa                                                 >  1:506763/1‑90 (MQ=255)
                 tCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTaa                                                 <  1:93948/90‑1 (MQ=255)
                        gtgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTTTACTCTGCACCCCCCCGGGaaagcaagg                                          >  2:344262/1‑90 (MQ=255)
                         tgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTTTATTTTTACTCTTCCCCCCCCCGGGaaaggaaggc                                         >  1:379394/1‑90 (MQ=255)
                         tgGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACTCTGCACCCCCCCGGGaaagcaaagc                                         >  2:382613/1‑90 (MQ=255)
                            aTTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTGACTCTGCACCCCCCCGGGAAGGCAAGGCAcc                                      >  2:62735/1‑90 (MQ=255)
                              tCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTTGACTCTGCACCCACCGGGGAAGGCAAGGCACcgc                                    >  2:137346/1‑90 (MQ=255)
                                          aCGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCct                        <  1:137346/90‑1 (MQ=255)
                                              tCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTTACCTTGCACCCCCCCGGGGAGGCAAGGGCACGCGCTTTTTCGCCCCTTc                    >  1:456047/1‑90 (MQ=255)
                                                cGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCa                  <  2:379394/90‑1 (MQ=255)
                                                                gagCGGCGGGATTTTATATTTCGACTCTGCACCCCCCGGGGAAGGGAAGGCACCGCGCTTTTTCGCCTCTTCCAGCACTCTGGCGGGaaa  >  2:532609/1‑89 (MQ=255)
                                                                                     |                                                                    
TGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGACTCTGCACCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAT  >  NZ_CP009273/3498362‑3498515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑