| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,734,269 | 0 | T | G | 54.5% | 12.4 / 25.8 | 22 | V402G (GTG→GGG) | avtA | valine‑‑pyruvate transaminase |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (12/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734184‑3734358 | ccAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAg > 1:443222/1‑90 (MQ=255) gggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt > 2:281430/1‑90 (MQ=255) gggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGAtt > 1:273557/1‑90 (MQ=255) ggATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGc > 2:279362/1‑90 (MQ=255) ggATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGc > 2:105362/1‑90 (MQ=255) aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCGGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAag > 1:66913/1‑90 (MQ=255) aCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGTGGAaga < 2:183685/90‑1 (MQ=255) ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGGAGAGATaa > 1:157702/1‑89 (MQ=255) aCGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGcc < 1:281430/90‑1 (MQ=255) cGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCt < 1:229558/90‑1 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTTTGGCGGGAGAGATAGAAAGAGCCTGGGCTGAAAggc > 1:448819/1‑88 (MQ=255) tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAggc > 1:72328/1‑88 (MQ=255) aCTACGTACCAGAGCCGGGGAAAATTGAGGCGGGGGGGGAGATTTTGGCGGGAGAGATAGAAAAAGCCGGGGGGGGAAGTAACGAAAAAt > 1:150185/1‑90 (MQ=255) agagCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgc > 2:351164/1‑90 (MQ=255) gagCCGGAGAAAATTGAGGCGGGGGGGAAGATTCTGGCGGAAGAGATAGGAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg > 2:185864/1‑90 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 1:98811/90‑1 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 1:527104/90‑1 (MQ=255) agCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcgc < 2:105927/90‑1 (MQ=255) gagaAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGc < 2:7702/90‑1 (MQ=255) agaAAATTGAGGCGGGGGGGAAGGGTTTGGCGGAAGAGAAAGAAAAAGGCTGGGCTGAAAATGACTAAAAATTTACGGGTGCGCTATGCt > 1:518598/1‑90 (MQ=255) ttGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAg < 1:73580/90‑1 (MQ=255) ggggggggAGATTGTTGCGGCAGAGATAAAAAAAGGCTGGGGTGCAATTTCCTAAAAAATTCCTGATCCGCCACGCTTATCAGGcccata > 1:452864/1‑86 (MQ=255) gggggTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa > 2:394424/1‑90 (MQ=255) tGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtt < 1:31571/90‑1 (MQ=255) tGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtt < 2:347357/90‑1 (MQ=255) | CCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATT > NZ_CP009273/3734184‑3734358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |