| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,946,904 | 0 | C | G | 53.8% | 9.4 / 15.3 | 13 | R23G (CGC→GGC) | ilvD | dihydroxy‑acid dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base G (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCA > NZ_CP009273/3946823‑3946986 | gggAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCGTGCGCCGTGGGGCGCcccc > 2:192998/1‑90 (MQ=255) gTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGGGCCGTGGCGCGCcccccgga > 2:76157/1‑86 (MQ=255) gTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCCCATGGGGGGTGGGGCGCcccccgca > 2:285079/1‑86 (MQ=255) ataaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGGGCGCCGTGGGGGGCCCCCCGCATGAc > 2:335458/1‑90 (MQ=255) aaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCg < 1:335458/90‑1 (MQ=255) tGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGGGGTGTGGGGGGGCCCCCGAAAGAACGACCCCg > 2:59007/1‑90 (MQ=255) tGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGCGCCGGGGGGGGCCCCCCGGATGAACgccgccc > 2:225928/1‑89 (MQ=255) tGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCGCGGGCGGTGGGGCGCCCCCCGGGTGACCCccgccg > 1:97565/1‑90 (MQ=255) accacTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATcgcg < 2:18426/90‑1 (MQ=255) gTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGt < 2:310768/90‑1 (MQ=255) gTAATATGGCGGGGGCTCCTGCGCCGTGGGGCGCCCCCCGGAAGACCCACGCCCCCTTCTGTAAGCCGATTATCGCGGGTGTGGAATCGt > 2:532543/1‑90 (MQ=255) gcTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCa > 1:519241/1‑90 (MQ=255) gcTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCa > 2:242917/1‑90 (MQ=255) | GGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCA > NZ_CP009273/3946823‑3946986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |