Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,195,5490GT54.5% 7.2 / 16.5 11S104* (TCA→TAA) purDphosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base T (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCAC  >  NZ_CP009273/4195465‑4195622
                                                                                    |                                                                         
aGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAAAGCTTTTTAGCCt                                                                      >  2:141761/1‑90 (MQ=255)
  cgcAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAAACTTTGGGAAACGCTTTTTAGCCTTc                                                                    >  2:5210/1‑90 (MQ=255)
                cGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATTTTTTTTTAAAGCTTTTTAGCCTTCCAGTTTGGCCGGAc                                                      >  2:522202/1‑90 (MQ=255)
                 ggTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTTGTAAAAGCTTTTTTGCCTTCCAGTTGGGGCGcccc                                                     >  2:466918/1‑90 (MQ=255)
                            ggTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTTGTAAAAGCTTTTTAGCCTTCCAGTTGGGCCGCACCTGCGGTTgggg                                          >  2:48893/1‑89 (MQ=255)
                                 tCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAg                                     <  2:11581/90‑1 (MQ=255)
                                                ttATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCcggcg                      <  1:102724/90‑1 (MQ=255)
                                                       gggCCAGGAAATCTTTGGTAAACGCTTTTTGGCCTTCCAGTTGGGCCGCACCTGCGGGTGGGGCGAAGATTTTTAGCCCcgcggcgcggg               >  1:197425/1‑89 (MQ=255)
                                                        ggCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGaa              <  1:5210/90‑1 (MQ=255)
                                                                    tttGGTAAACGCTTTTGGGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCAc  <  2:197425/90‑1 (MQ=255)
                                                                    tttGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCAc  <  1:151858/90‑1 (MQ=255)
                                                                                    |                                                                         
AGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCGACCAC  >  NZ_CP009273/4195465‑4195622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑