Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,912	G→T	W30C (TGG→TGT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,912	0	G	T	100.0%	56.4 / NA	18	W30C (TGG→TGT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/9);  total (9/9)

GATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49834‑49991                                                                               |                                                                                gATTGCGGCTTTAGCGGTAGACCGCTTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                      <  1:343482/90‑1 (MQ=255)  atgccggcgTTAGCGGTCCATCCCGTTATCGGCATGCAAAACGCCCTGCCGTGGATCCTGCCTGCCGATCTCGCCCGTTTTAAACGCAac                                                                     <  2:332041/86‑1 (MQ=255)  aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                     <  2:169127/90‑1 (MQ=255)      cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt                                                                 <  1:429913/90‑1 (MQ=255)      cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt                                                                 <  1:485132/90‑1 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                >  2:11859/1‑90 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                >  2:388492/1‑90 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                >  1:404379/1‑90 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCAAGCCGCGGAACCCGCCTGCCGATCTCGGCTGTTTTAAACGCCACAACtt                                                                >  1:399682/1‑90 (MQ=255)               cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAc                                                        <  2:404379/90‑1 (MQ=255)                        gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  1:531604/1‑90 (MQ=255)                                   aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                              >  1:486944/1‑80 (MQ=255)                                   aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                              <  2:486944/80‑1 (MQ=255)                                               aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                        >  1:155750/1‑90 (MQ=255)                                                tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                       >  2:413850/1‑90 (MQ=255)                                                      ggAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                 <  2:123958/90‑1 (MQ=255)                                                                 gccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa      >  1:405000/1‑90 (MQ=255)                                                                     aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  2:67923/90‑1 (MQ=255)                                                                               |                                                                                GATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49834‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑