Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,840,0330TG53.8% 9.2 / 14.0 13T247P (ACC→CCC) hycDformate hydrogenlyase subunit HycD
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (7/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGAT  >  NZ_CP009273/2839959‑2840102
                                                                           |                                                                     
gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCa                                                        <  1:16245/90‑1 (MQ=255)
gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCa                                                        <  2:149560/90‑1 (MQ=255)
 aTAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCAc                                                       <  1:105345/90‑1 (MQ=255)
           cGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCACGGAATaaaac                                             >  2:16245/1‑88 (MQ=255)
             ccGACCACCAGTTTTACGGTGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCACGGAATAAAAAcc                                           >  1:58684/1‑90 (MQ=255)
             ccGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTTCCCACCGAATAAACAcc                                           >  2:142132/1‑90 (MQ=255)
              cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCCCGGGATAAAAAccc                                          >  1:359045/1‑90 (MQ=255)
                    ccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCcgacga                                    <  2:341604/90‑1 (MQ=255)
                         tttACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTCCATTTTTCCCCCCGGAATAAACACCCCCACGAACATc                               >  1:344600/1‑90 (MQ=255)
                                 ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGGAAGGTTT‑CATTTTTCCCCCCCGGAAAAAAACCCCCACGAACAACTGCAacac                       >  2:206501/1‑90 (MQ=255)
                                        aCCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGc                <  2:58684/90‑1 (MQ=255)
                                                 agcagcagTCCACCGGCGGG‑GAAGGGTTCCATTTTTCCCCCCGGAATAAACACCCCCACGAACATCTTCAACACCACCAGCTGTTTCAgg       >  1:245007/1‑90 (MQ=255)
                                                      cagTCCACCGGCGGT‑GGAGGGATCCATTTTTTCCCACGGAAAAAACACCCCCACGTACAACTGCAACACCACCAGCTGCTTCACGCTgtc  >  2:304994/1‑88 (MQ=255)
                                                                           |                                                                     
GATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGAT  >  NZ_CP009273/2839959‑2840102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑