| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,840,042 | 0 | G | T | 53.3% | 15.3 / 26.4 | 15 | Q244K (CAA→AAA) | hycD | formate hydrogenlyase subunit HycD |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGAT > NZ_CP009273/2839959‑2840102 | gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCa < 1:16245/90‑1 (MQ=255)gATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCa < 2:149560/90‑1 (MQ=255) aTAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCAc < 1:105345/90‑1 (MQ=255) cGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCACGGAATaaaac > 2:16245/1‑88 (MQ=255) ccGACCACCAGTTTTACGGTGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCACGGAATAAAAAcc > 1:58684/1‑90 (MQ=255) ccGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTTCCCACCGAATAAACAcc > 2:142132/1‑90 (MQ=255) cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTTCATTTTTCCCCCCGGGATAAAAAccc > 1:359045/1‑90 (MQ=255) ccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCcgacga < 2:341604/90‑1 (MQ=255) tttACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGGTTCCATTTTTCCCCCCGGAATAAACACCCCCACGAACATc > 1:344600/1‑90 (MQ=255) ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGGAAGGTTT‑CATTTTTCCCCCCCGGAAAAAAACCCCCACGAACAACTGCAacac > 2:206501/1‑90 (MQ=255) aCCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGc < 2:58684/90‑1 (MQ=255) agcagcagTCCACCGGCGGG‑GAAGGGTTCCATTTTTCCCCCCGGAATAAACACCCCCACGAACATCTTCAACACCACCAGCTGTTTCAgg > 1:245007/1‑90 (MQ=255) cagTCCACCGGCGGT‑GGAGGGATCCATTTTTTCCCACGGAAAAAACACCCCCACGTACAACTGCAACACCACCAGCTGCTTCACGCTgtc > 2:304994/1‑88 (MQ=255) tGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGc > 1:274497/1‑48 (MQ=255) tGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGc < 2:274497/48‑1 (MQ=255) | GATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGT‑GAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGAT > NZ_CP009273/2839959‑2840102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |