| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,348,814 | 0 | T | C | 55.6% | 14.8 / 21.5 | 18 | I244T (ATC→ACC) | gltB | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base C (0/10); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTC > NZ_CP009273/3348744‑3348902 | aaCACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATgg > 1:135913/1‑90 (MQ=255) acCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcg > 1:42575/1‑90 (MQ=255) ccGTCCCGCGCGGCCCGCTGGCGCACCCGTCCCGCTATCGGGCGCATAACGGTAAAACCACCCCCACCCCCGGTAACCGCCAATGGgcgc < 1:76369/90‑1 (MQ=255) gTACGGCGCCGGCCGCCGGCGCAACCGTTGCGTTATGTGGCGCGTAACGGTGAGATCAACACCATCACCGGTAGCCGCCAATGGgcgcgt > 2:187302/1‑90 (MQ=255) cgcgcgGGCCGCGGGCCCAACCGTCCCGCTATCGGGCGCATAAGGGAGAAACAAACACCACCCCCGGTAACCGCCAATGGgcgcgtgcgc < 1:346536/90‑1 (MQ=255) ccggctggccgctggccCACCCTTTCCTTTACCTGGCGAAAACCGGCAAAAACACCCCCACCCCCGATAACCGCCAATGGgcgcgtgcgc < 2:215143/87‑1 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcgcg > 1:215143/1‑90 (MQ=255) ccacccGTCCCGCTATCGGGCGCAAAAGGGTAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCa < 2:135913/86‑1 (MQ=255) cGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGacac > 1:240866/1‑90 (MQ=255) cGTCCCGTTTTCTGGCGCAAAAGGGAAAAATCAACCCCACCACGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGacac < 1:212088/90‑1 (MQ=255) cGCTTTCTGGCGCATAACGGTGAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 1:24359/90‑1 (MQ=255) cGCTTTCGGGCGCATAACGGTAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 1:246973/90‑1 (MQ=255) cGCTATCGGGCGCATACGGGTAAAACCACCCCCACCCCCGGAACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt < 2:344977/90‑1 (MQ=255) tcgggCGCAAAAGGGTAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 2:136476/87‑1 (MQ=255) cgcATAACGGAAAAACCAACCCCCCCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGAcc < 2:42575/90‑1 (MQ=255) gTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacgacg > 1:206459/1‑90 (MQ=255) aaCACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCg > 2:206507/1‑90 (MQ=255) aTCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTc > 2:44267/1‑90 (MQ=255) | AACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTC > NZ_CP009273/3348744‑3348902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |