| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,432 | 0 | A | C | 64.3% | 8.3 / 11.1 | 14 | T114T (ACA→ACC) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/9); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCC > NZ_CP009273/3806347‑3806519 | ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTg < 2:253140/90‑1 (MQ=255) cTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAg > 1:276134/1‑90 (MQ=255) aCGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTg > 1:329313/1‑90 (MQ=255) ccGTTTTGCGGCCGGAATGGCGAATGCCCTGGTATCGCCGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACca < 1:255215/90‑1 (MQ=255) ccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTGGCCGTGCCCCCCGCCATGAACca < 1:291798/90‑1 (MQ=255) gtgtTGCTGCGGGAAGGGCGAATGCCCTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACcagc < 2:144508/90‑1 (MQ=255) ctgcGGGAAGGGCGAATGCCCTGGTATCGACGATTTGTCGGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGt < 2:81607/90‑1 (MQ=255) tGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGGGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCg > 2:263029/1‑90 (MQ=255) cccTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACgc < 1:100885/89‑1 (MQ=255) aCCTGGTCTCGACGTTTTGTCGGCCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACgc < 2:41893/90‑1 (MQ=255) gTACCGCCGTTTTTTCTGGCCACCCCGGCCCCTGTACCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt < 2:170477/90‑1 (MQ=255) cgttttGTCTGGCTACCCCTGCCCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTag < 2:311320/87‑1 (MQ=255) aTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTagag > 1:46813/1‑90 (MQ=255) tttGTCTGGCCACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAgg < 1:304034/90‑1 (MQ=255) acacCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTcc > 2:219316/1‑90 (MQ=255) | TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCC > NZ_CP009273/3806347‑3806519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |