| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,169 | 0 | T | G | 53.3% | 5.9 / 17.3 | 15 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (8/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCG > NZ_CP009273/937083‑937256 | cAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAc > 1:92047/1‑90 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:328736/90‑1 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:84681/90‑1 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc < 1:136581/90‑1 (MQ=255) aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc < 2:92047/90‑1 (MQ=255) gtgaGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGa < 2:274702/87‑1 (MQ=255) gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAAACTAATGcccc > 1:101612/1‑89 (MQ=255) tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCCCATg > 1:24421/1‑90 (MQ=255) gTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGGACGCCAGAAAACTAATGCCCGCATGatca > 1:38183/1‑90 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAAACTAATGCCCGCCTGatcatcatc > 2:9685/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 1:170288/90‑1 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAAACTAATGCCCGCATGATTATCATCGATcc > 1:329929/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTAATATCTTGAAAAAGGACGCCCGAAAAATAAAGCCCCCCAGATTAACAACGATcc > 2:266126/1‑90 (MQ=255) aCGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAAATTAATGCCCCCCTGGTCATCATCGATCCCCGCtat > 2:66371/1‑90 (MQ=255) ggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcg < 1:26459/90‑1 (MQ=255) | CAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCG > NZ_CP009273/937083‑937256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |