| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,074,570 | 0 | T | G | 53.8% | 7.5 / 12.3 | 13 | T58T (ACA→ACC) | sbmC | DNA gyrase inhibitor SbmC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACT > NZ_CP009273/2074482‑2074649 | cTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtg > 1:49394/1‑90 (MQ=255) tCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGttt > 1:237571/1‑90 (MQ=255) gAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGGTTcct > 2:182943/1‑88 (MQ=255) gCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGAtt < 2:60110/90‑1 (MQ=255) gAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGACAGCaa > 2:247033/1‑90 (MQ=255) aGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGACAGCAAcc > 1:269943/1‑90 (MQ=255) gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTTTCGTAATAGACAGCAAccc > 1:366920/1‑90 (MQ=255) tAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTc < 2:237571/90‑1 (MQ=255) gCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACaa > 2:109872/1‑90 (MQ=255) aCCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGAAAGCAACCCACCGCTTTGGCACAata > 2:327382/1‑90 (MQ=255) gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca < 1:327382/90‑1 (MQ=255) gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca < 2:31847/90‑1 (MQ=255) gCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCCCTCCTTCGGCACAAAATTTTTTCTATCTACCCAcat > 2:229540/1‑90 (MQ=255) tttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACt < 1:247033/90‑1 (MQ=255) | CTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACT > NZ_CP009273/2074482‑2074649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |