Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,383	Δ1 bp	coding (7/435 nt)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,380	0	A	.	100.0%	43.1 / NA	11	coding (4/435 nt)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/5);  total (6/5)

TGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGC  >  NZ_CP009273/1613326‑1613457                                                       |                                                                              tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                           >  1:242246/1‑90 (MQ=255) tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                           >  2:148131/1‑90 (MQ=255) tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                           >  2:182192/1‑90 (MQ=255) tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                           >  2:83508/1‑90 (MQ=255)      gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGt                                      <  1:148131/90‑1 (MQ=255)      gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGt                                      <  2:164939/90‑1 (MQ=255)           atatTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCtt                                 <  1:24089/90‑1 (MQ=255)           atatTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCtt                                 >  2:227875/1‑90 (MQ=255)                  ccccTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                          <  1:227875/90‑1 (MQ=255)                                    tGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGaagaaa        >  2:261553/1‑90 (MQ=255)                                          ggCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGc  <  1:83508/90‑1 (MQ=255)                                                       |                                                                              TGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGC  >  NZ_CP009273/1613326‑1613457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑