| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,300,628 | 0 | T | G | 53.8% | 9.8 / 13.9 | 13 | T198P (ACC→CCC) | deaD | ATP‑dependent RNA helicase DeaD |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACG > NZ_CP009273/3300542‑3300713 | aGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGaaa > 1:35924/1‑89 (MQ=255) tCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGc > 1:97351/1‑90 (MQ=255) tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCg > 1:111706/1‑90 (MQ=255) tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAAATGGACGAAACg > 1:192770/1‑90 (MQ=255) tGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCa > 1:50643/1‑90 (MQ=255) ggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAAACGCTTCCCGCATGGGTGCaga > 1:131798/1‑90 (MQ=255) cGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACagag < 2:111706/90‑1 (MQ=255) cTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCg < 1:39659/90‑1 (MQ=255) cTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCg < 1:98621/90‑1 (MQ=255) cgcACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCGTGGTTGCGGAGAACAGAGCGGGGTGATGAACt > 2:158156/1‑90 (MQ=255) ttCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAAGGGGGGCTGATGACCTTCCgg > 2:298338/1‑90 (MQ=255) tCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATaa < 2:35924/90‑1 (MQ=255) cggGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACg < 1:298338/90‑1 (MQ=255) | AGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACG > NZ_CP009273/3300542‑3300713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |