Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,884	C→A	P21Q (CCG→CAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,884	0	C	A	100.0%	15.2 / NA	7	P21Q (CCG→CAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/4);  total (3/4)

AAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49815‑49969                                                                      |                                                                                      aaaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGAtc                                                                   <  2:76202/90‑1 (MQ=255)       cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGACATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCt                                                             <  1:71725/90‑1 (MQ=255)                                                     cATGGAAAACGCCATGCAGTGGACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa               <  1:48926/90‑1 (MQ=255)                                                          aaaaCGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt          <  2:279493/90‑1 (MQ=255)                                                              cGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa      >  1:261790/1‑90 (MQ=255)                                                                  aTGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca  >  1:77217/1‑90 (MQ=255)                                                                  aTGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca  >  2:302663/1‑90 (MQ=255)                                                                      |                                                                                      AAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49815‑49969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑