Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,184,613	A→T	I207N (ATC→AAC)	phoQ ←	two‑component system sensor histidine kinase PhoQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,184,613	0	A	T	100.0%	20.3 / NA	8	I207N (ATC→AAC)	phoQ	two‑component system sensor histidine kinase PhoQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/4);  total (4/4)

TTATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAT  >  NZ_CP009273/1184529‑1184697                                                                                     |                                                                                     ttATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACt                                                                                 <  2:247864/90‑1 (MQ=255)  tATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTa                                                                                <  2:132548/90‑1 (MQ=255)              gTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTgg                                                                    >  2:15951/1‑90 (MQ=255)                    ggACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTgaga                                                              <  1:66928/90‑1 (MQ=255)                                   gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc                                               >  2:295438/1‑90 (MQ=255)                                                          caccaGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCGGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACtt                        >  1:211584/1‑90 (MQ=255)                                                                gcggcgACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGc                  >  1:165109/1‑90 (MQ=255)                                                                                gcGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAt  <  1:234212/90‑1 (MQ=255)                                                                                     |                                                                                     TTATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAATGGTAT  >  NZ_CP009273/1184529‑1184697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑