Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,851,887	G→A	R479H (CGT→CAT)	mutS →	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,851,887	0	G	A	100.0%	50.0 / NA	15	R479H (CGT→CAT)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

GTCCGCGAGCGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCAGA  >  NZ_CP009273/2851805‑2851965                                                                                   |                                                                               gTCCGCGAGCGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCaa                                                                         <  2:199895/90‑1 (MQ=255)      cgAGCGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCa                                                                    <  1:9070/90‑1 (MQ=255)            tGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGc                                                              <  2:61118/90‑1 (MQ=255)                      ggCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAAc                                                    >  1:65028/1‑90 (MQ=255)                       gCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACt                                                   >  2:102594/1‑90 (MQ=255)                                cGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCATCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTc                                          >  1:138308/1‑90 (MQ=255)                                 gCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCg                                         >  1:117808/1‑90 (MQ=255)                                 gCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCg                                         >  1:12842/1‑90 (MQ=255)                                          tGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCt                                >  1:292427/1‑90 (MQ=255)                                                                tactacATTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTAcatc          <  1:139449/90‑1 (MQ=255)                                                                      aTTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCa    <  1:34729/90‑1 (MQ=255)                                                                      aTTCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCa    >  2:166470/1‑90 (MQ=255)                                                                        tCAAATCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCaga  >  1:226367/1‑90 (MQ=255)                                                                           aaTCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCa    <  1:282194/85‑1 (MQ=255)                                                                           aaTCAGCCATGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCa    >  2:282194/1‑85 (MQ=255)                                                                                   |                                                                               GTCCGCGAGCGTGAACGTACCGGCCTGGACACGCTGAAAGTTGGCTTTAATGCGGTGCACGGCTACTACATTCAAATCAGCCGTGGGCAAAGCCATCTGGCACCCATCAACTACATGCGTCGCCAGACGCTGAAAAACGCCGAGCGCTACATCATTCCAGA  >  NZ_CP009273/2851805‑2851965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑