Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,898	G→A	A26T (GCC→ACC)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,898	0	G	A	100.0%	48.7 / NA	16	A26T (GCC→ACC)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

GAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT  >  NZ_CP009273/49814‑49957                                                                                     |                                                            gAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAACTGCCTACCGAt                                                        >  1:271772/1‑90 (MQ=255)  aaaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGAtc                                                       <  2:35516/90‑1 (MQ=255)               cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTaa                                          >  1:66529/1‑90 (MQ=255)                     gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCaa                                    <  2:135906/90‑1 (MQ=255)                     gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCaa                                    <  2:66529/90‑1 (MQ=255)                      aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAac                                   >  1:326036/1‑90 (MQ=255)                      aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAac                                   <  1:328058/90‑1 (MQ=255)                         gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACAcc                                >  2:179103/1‑90 (MQ=255)                           ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCtt                              >  1:263833/1‑90 (MQ=255)                           ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCtt                              >  2:179699/1‑90 (MQ=255)                                               ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg          <  1:174053/90‑1 (MQ=255)                                               ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg          <  1:362161/90‑1 (MQ=255)                                                       aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt  <  1:370819/90‑1 (MQ=255)                                                       aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt  <  1:373086/90‑1 (MQ=255)                                                                   aTGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCaa                                    >  1:33031/1‑44 (MQ=255)                                                                   aTGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCaa                                    <  2:33031/44‑1 (MQ=255)                                                                                     |                                                            GAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAT  >  NZ_CP009273/49814‑49957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑