Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	452,311	A→T	Q60L (CAG→CTG)	clpP →	ATP‑dependent Clp endopeptidase proteolytic subunit ClpP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	452,311	0	A	T	100.0%	32.2 / NA	10	Q60L (CAG→CTG)	clpP	ATP‑dependent Clp endopeptidase proteolytic subunit ClpP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/6);  total (4/6)

TGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGC  >  NZ_CP009273/452266‑452389                                              |                                                                               tGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGAtatctatc                                    <  1:107575/90‑1 (MQ=255)  gACTGGCCAGGTTGGAGACCACATGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATatctatct                                   >  2:258164/1‑90 (MQ=255)   aCTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTg                                  <  2:231005/90‑1 (MQ=255)              tGAAGACCACATGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTc                       >  2:75995/1‑90 (MQ=255)                      acaTGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCg               <  1:204009/90‑1 (MQ=255)                       caTGGCTAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCgg              <  1:258164/90‑1 (MQ=255)                             tAACCTGATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGAt        <  2:104314/90‑1 (MQ=255)                                   gATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTa                           >  1:240456/1‑65 (MQ=255)                                   gATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTa                           <  2:240456/65‑1 (MQ=255)                                   gATTGTGGCGCTGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGc  >  2:294843/1‑90 (MQ=255)                                              |                                                                               TGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGC  >  NZ_CP009273/452266‑452389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑