| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 216,602 | 0 | A | C | 54.5% | 5.4 / 10.9 | 11 | *272E (TAA→GAA) | metQ | methionine ABC transporter substrate‑binding lipoprotein MetQ |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACG > NZ_CP009273/216519‑216690 | gAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGcc > 2:286276/1‑90 (MQ=255) taaaaaaaTGACAAGACGGGCAAGCCCCCGCCCTAAATGATATTACAAATTGTGGAAACAGCCAAAAAATTCCCAGCCTTTAACACCTcc < 1:286276/87‑1 (MQ=255) tGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTCCCAGCCTTTAACAGCTCCGCCGTTaa < 2:319076/90‑1 (MQ=255) cgcCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTCCCAGCCTTTAACAGCTccgc < 2:283965/68‑1 (MQ=255) cgcCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTccgc > 1:283965/1‑68 (MQ=255) taaCAAATTGTGAAAACAGCCAAAAAATTCCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGa < 1:41034/88‑1 (MQ=255) taaaaaTTGTGGAAACAGCCTAAAAATTCCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGAc < 2:188951/87‑1 (MQ=255) aaTTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGAt > 2:261756/1‑90 (MQ=255) ttGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATaa > 2:308583/1‑90 (MQ=255) tAAAAATTCCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAAttt < 2:130565/90‑1 (MQ=255) tACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACg > 2:206782/1‑90 (MQ=255) | GAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACG > NZ_CP009273/216519‑216690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |