Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273390,7450AC58.3% 5.7 / 10.6 12G333G (GGT→GGG) ampHD‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase AmpH
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATC  >  NZ_CP009273/390672‑390828
                                                                          |                                                                                   
ccAACGGCAAACGAGTGCCCCCCAAAACCCCCCAAATTTTTTTCTGGGGGATCACCCCCATATAGGTAATAAA‑CCCGCCACCACCGCCTg                                                                     <  1:336701/89‑1 (MQ=255)
     ggCAAAGGAGTGACCCCCACAACCCCCCAAATTTTCTCCGGGGGATTCACCCCAATATGGGTATAAAA‑CCCGCCACCACCGCCTGtcttc                                                                <  1:346750/90‑1 (MQ=255)
          aCGAGTGCCCCCCAAAACCCCCCCAATTTTCTTCTGGGGGATCATCCCCATATAGGTAAAAAA‑CCCGCCACCACCGCCTGTCTTCTGaat                                                           <  1:123747/90‑1 (MQ=255)
                       acaAACGCCCAAATTTCCTCCGGGGGGATCACCGCCATAATGATAATAAA‑CCCCCCCCCACCGCCTGTCTTCTGAATAATCCCCGgacga                                              <  1:152478/90‑1 (MQ=255)
                          aaCGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGAccc                                           >  2:30681/1‑90 (MQ=255)
                           aCGCCCAAATTTTTTTCTGGGGGATCACCCCCATAAAGAAAATAAA‑CCCCCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCct                                          <  2:220159/90‑1 (MQ=255)
                                      ttcttcTGCGGACTCACCCCCATA‑AGGTAAACAACCCCGCCCCCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGcc                               <  1:177450/90‑1 (MQ=255)
                                        cttctGCGGGATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCat                             >  2:253010/1‑90 (MQ=255)
                                        cttctGCGGGATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCat                             >  2:291628/1‑90 (MQ=255)
                                          tctGCGGAATCACCGCCAAATAGGTAATAAA‑CCCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCatat                           <  2:178284/90‑1 (MQ=255)
                                               gggATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc                      >  2:77786/1‑90 (MQ=255)
                                                                   aaTGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATc  >  1:243035/1‑90 (MQ=255)
                                                                          |                                                                                   
TCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAA‑ACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATC  >  NZ_CP009273/390672‑390828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑