Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0030TC54.5% 7.9 / 12.2 11intergenic (+118/+15)gsk/ybaLinosine/guanosine kinase/Kef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (0/6);  total (2/9)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGC  >  NZ_CP009273/496922‑497087
                                                                                 |                                                                                    
gccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAac                                                                              <  1:93289/90‑1 (MQ=255)
  cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAacca                                                                            <  2:174150/90‑1 (MQ=255)
            aTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                                  <  2:3521/90‑1 (MQ=255)
             tttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcccc                                                                 >  1:340258/1‑90 (MQ=255)
                                              gttttATCATCCCTCCATTTTTTTCATTTTTTACTCCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                <  1:135253/87‑1 (MQ=255)
                                              gttttATCATCCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc                                <  2:274322/87‑1 (MQ=255)
                                              gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                >  1:361358/1‑90 (MQ=255)
                                              gTCTTATCACCCCTACATTTTTTTCATATTTTCACCCCCCCCCCCGCCGTTCCCCCCCCCCCCCCCACCCCCGCCGGTGGCGTTTCcagc                                <  1:288616/90‑1 (MQ=255)
                                                         ccTACTTTTTTTTCATTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                     <  2:250384/90‑1 (MQ=255)
                                                         ccTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCGTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                     <  1:78068/90‑1 (MQ=255)
                                                                            ttACACCCGACACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc  <  2:203420/90‑1 (MQ=255)
                                                                                 |                                                                                    
GCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGC  >  NZ_CP009273/496922‑497087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑