Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3470TA54.5% 3.1 / 10.2 11intergenic (+37/‑66)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base A (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA  >  NZ_CP009273/2380265‑2380405
                                                                                  |                                                          
tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACatt                                                     <  2:285364/90‑1 (MQ=255)
               tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGTCATGCACATTTTATTTTCCCTCATc                                      <  2:242115/90‑1 (MQ=255)
                        tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAAGGACAAGCACAATTTTTTTTTCCTCATTTTTTTTTAg                             >  2:148636/1‑90 (MQ=255)
                        tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAGGAGAAACACATTTTTTTTTTCCTCCTCtttttttta                             >  2:238044/1‑87 (MQ=255)
                                acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAAAAAAGGACAATTTATTTTTTCTCCTCTGTTTTTAGAAGCGgaa                     >  1:171975/1‑90 (MQ=255)
                                 caacaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGgaaa                    >  2:74643/1‑89 (MQ=255)
                                     aGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGAAAAAtaa                >  2:218327/1‑88 (MQ=255)
                                      gTAAATAATAAAAACTTCTCGAGGCGGGGCCCGAAAAAAAGAAAATCACAATTTTTTTTTCCCCCTCTGTTTTTAAATGGGAAAAAAGaa               >  1:146502/1‑90 (MQ=255)
                                             ataaAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGGGAAAAATGAAAATTAAc        >  2:233729/1‑90 (MQ=255)
                                               aaaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgg      <  1:233729/90‑1 (MQ=255)
                                                   aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga  <  2:171975/90‑1 (MQ=255)
                                                                                  |                                                          
TCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA  >  NZ_CP009273/2380265‑2380405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑