| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,659,062 | 0 | C | A | 63.6% | 0.9 / 13.1 | 11 | A37S (GCG→TCG) | gadX | acid resistance transcriptional activator GadX |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base A (0/7); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAT > NZ_CP009273/3658978‑3659151 | tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa > 2:156321/1‑90 (MQ=255) cccTTGACACAAAAAAAAATTTTTCACCCCCCTTTTCTCCTCAATTTTGGTTTCCACCCAAAAAACCCAGGTCCCCGCCATTAAAATAGc < 1:357686/90‑1 (MQ=255) cacGAATAAAAAATTTTCAACACCCTTATCTCCTCAATTTTGGTTTGCACCCCAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTc < 2:103792/90‑1 (MQ=255) taaaCACTTTTATCTCCTCGATTTTGGTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 2:249580/88‑1 (MQ=255) aaCACCCTTATTTACTAAATTTTGGTTTCCACCCAAAAAAACCAGCCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag < 1:347073/90‑1 (MQ=255) cacccTTATCTCCTAAATTTTGGTTTCCACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa < 1:129482/90‑1 (MQ=255) ttGGCTTGCATCCGCAAAAACCAGGTCACCACCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa > 1:270220/1‑90 (MQ=255) tGGCTTGCACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc < 2:138393/90‑1 (MQ=255) tGCACCCGAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt < 2:69390/90‑1 (MQ=255) gCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt > 1:74716/1‑90 (MQ=255) cGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa > 1:242710/1‑90 (MQ=255) cAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt < 2:74716/90‑1 (MQ=255) | TCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAT > NZ_CP009273/3658978‑3659151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |