Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927337,3380TG53.8% 6.1 / 11.5 13T163P (ACC→CCC) caiCcrotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTCA  >  NZ_CP009273/37253‑37409
                                                                                     |                                                                       
acacCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCa                                                                     >  2:15070/1‑90 (MQ=255)
acacCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCa                                                                     <  2:53230/90‑1 (MQ=255)
     tttCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGggtg                                                                >  2:191035/1‑90 (MQ=255)
     tttCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGggtg                                                                >  2:203301/1‑90 (MQ=255)
        cggtcggGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGttgttg                                                             <  2:102009/90‑1 (MQ=255)
                        ccGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCAAAAGGGGGCCGGGTGTTGATTTTTTAGTTGAGTa                                             >  2:187907/1‑90 (MQ=255)
                                     gaATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGc                                >  1:107907/1‑90 (MQ=255)
                                          tCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGGTGTTGATTTTTCAGTTGAGTAAACGAACTCCCGCcatca                           >  2:350209/1‑90 (MQ=255)
                                                    cGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGaa                 <  1:134378/90‑1 (MQ=255)
                                                    cGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGaa                 <  2:107907/90‑1 (MQ=255)
                                                        aGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGTGc             >  2:333296/1‑90 (MQ=255)
                                                            gATAGCGGCGGTGCATAGCACAAGGGGGGAGGTTGTTGATTTTTCAGTTGAGTAAACGAAGTCACGCCATCATCAGCGGGAAGTGCcaca         >  2:199918/1‑90 (MQ=255)
                                                                   gcggTGCATAGCACAAGGGGGCAGGTTGTTGATTTTTCAGTTGAGGAAACGAACTCCCGCCATCATCAGCGGGGAGTGCCACATCTGTCa  >  1:156860/1‑90 (MQ=255)
                                                                                     |                                                                       
ACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTCA  >  NZ_CP009273/37253‑37409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑