| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 117,994 | 0 | A | T | 53.3% | 5.2 / 17.0 | 15 | L15L (CTT→CTA) | aroP | aromatic amino acid transporter AroP |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base T (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATG > NZ_CP009273/117905‑118081 | ccTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGAGATAAGCTGAATATGGCGGTTTTTa < 1:260677/90‑1 (MQ=255) aCGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGCCGCGCTTTTgc > 2:163991/1‑90 (MQ=255) gAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTtgctgc > 2:214295/1‑90 (MQ=255) ggCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTTGCTGCTc > 2:219103/1‑90 (MQ=255) ggCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGGTTTTTAAGCCGCGCCTTTgctggct > 1:175312/1‑87 (MQ=255) gcTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTTGCTGCTCGcc > 2:10578/1‑90 (MQ=255) ggTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTccct > 2:22568/1‑88 (MQ=255) ggTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCcat < 1:163991/90‑1 (MQ=255) aTAAGCTGAATATGGCGGGTTTTTAAGCCGCGCTTTAGCCGCTCCCCGGGCTGTTGGCCTTCCCTCATTAAAACTCGTGCGGGGggtggt > 1:247536/1‑88 (MQ=255) gCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGtttttt < 1:109021/90‑1 (MQ=255) gCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGtttttt < 2:355010/90‑1 (MQ=255) tGGCGGTTTTTTAGGCCGCGCTTTTGCTGCTCGCCGGGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTTATCTAc > 1:5149/1‑90 (MQ=255) gCGGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGGGCTGTTGGCCTTCCATCATGAAAACTCGTGCGGGGGTTGGTTTTTTTTTCTTCGc > 1:317164/1‑90 (MQ=255) cGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCa < 1:185666/90‑1 (MQ=255) cGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACC‑TCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAg < 1:361445/90‑1 (MQ=255) ttAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATg < 1:187699/90‑1 (MQ=255) | CCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATG > NZ_CP009273/117905‑118081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |