Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,101,6750GT60.0% ‑0.7 / 10.8 10N156K (AAC→AAA) yiiQYiiQ family protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (0/6);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 3.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTG  >  NZ_CP009273/4101605‑4101760
                                                                      |                                                                                     
tCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTTTTTTTCCCCGCCGTtagta                                                                    <  2:105265/90‑1 (MQ=255)
       ttttcCGCGGTTGCCCGCGACAAAAAACCGCAGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTg                                                             <  2:287706/89‑1 (MQ=255)
        ttttCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc                                                            >  2:101765/1‑90 (MQ=255)
                              aaaaaCGCGGTCCCCCTTCTGTCTGGGTGTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGttt                                      <  2:294118/87‑1 (MQ=255)
                                   cGCAGTCCCCTTTCTGTCTGGGTTTAATATTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc                                 <  1:101765/90‑1 (MQ=255)
                                       gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa                             >  2:45778/1‑90 (MQ=255)
                                              ttCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                      >  1:137898/1‑90 (MQ=255)
                                               tctgtctgGGTTTAAAACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg                     <  2:257295/90‑1 (MQ=255)
                                               tctctcTGGGTTTAATTATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg                     <  1:315010/90‑1 (MQ=255)
                                                                aCGTTTGTTTTTCCCCGTCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGc    <  1:134667/90‑1 (MQ=255)
                                                                  gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg  >  1:156985/1‑90 (MQ=255)
                                                                  gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg  >  1:246804/1‑90 (MQ=255)
                                                                  gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg  >  2:366733/1‑90 (MQ=255)
                                                                      |                                                                                     
TCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTG  >  NZ_CP009273/4101605‑4101760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑