| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,215,417 | 0 | A | G | 54.5% | 5.2 / 12.0 | 11 | E198G (GAA→GGA) | ebgR | transcriptional regulator EbgR |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (6/0); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAA > NZ_CP009273/3215335‑3215500 | aaCCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGAAGAGCCCGGCAAAGGGGGTATTTGTGGGGGCGCCTTTGGGGGGTATGGc > 1:205572/1‑90 (MQ=255) ttAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGGGGGGATTCGGGGGGGTGCCTTTTCGGGAAATGGCCGGATGaaaa > 1:59925/1‑89 (MQ=255) ttAATCGTATTGGTTTTATTGGCGGGGAAGATGAGGCCGGCAAGGGGGGTATTTGTGGGGGCGCCCTTTCGGGATATTGCCGACTGaaaa > 1:77018/1‑89 (MQ=255) tATTGGTTTTATTGGCGGGGAAGATGAGCCTGGCAAGGGGGGTATTTTTGGGGGGGCCTTTTGGGGAAATGGGCGACTGAAAAAAGAggg > 1:231033/1‑89 (MQ=255) tttATTGGCGGTGAAGAAGAGCCCGGCAAAGCGGGTATTTGTGAGGGCGCCCTTTCGGGAAATTGCCGACTGAAAAAAAAGGGAcgcgga > 1:40435/1‑88 (MQ=255) aGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGGTTTTCCa > 1:293042/1‑90 (MQ=255) atatTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTtcgt < 1:66932/90‑1 (MQ=255) atTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTtcgtcg < 2:205572/90‑1 (MQ=255) tCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCggg > 2:266228/1‑90 (MQ=255) gTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGaa < 2:293042/90‑1 (MQ=255) ttGCGGGATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGGGTTTTCAATTTGGCGGGTTTAGAACTGGGaa > 1:169604/1‑90 (MQ=255) | AACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAA > NZ_CP009273/3215335‑3215500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |