Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,679,3810AC54.5% 2.5 / 11.2 11G1051G (GGT→GGG) bcsCcellulose synthase complex outer membrane protein BcsC
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTA  >  NZ_CP009273/3679309‑3679464
                                                                        |                                                                                   
ggtcggGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCa                                                                    <  1:328823/90‑1 (MQ=255)
           gATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgc                                                         >  1:69636/1‑90 (MQ=255)
                                         tGGTGCGTAAATGCGCCCACGAGCCAGACGCCCCCAGCCCCCCCGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACg                           <  2:102015/90‑1 (MQ=255)
                                          ggTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGa                          >  2:185187/1‑90 (MQ=255)
                                                       gcccACGACCCAGCCCCCCCCCCCCCCCCCGACCATTTTTCCGGGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGc             <  1:62856/88‑1 (MQ=255)
                                                       gACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGc             >  1:255360/1‑90 (MQ=255)
                                                          cccGAGCCAGCCGCCCCCAGCCCCCCCGCCCATTTTCCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa          <  2:336394/88‑1 (MQ=255)
                                                          cACGAGCCAGCCGCCCCCAGCCCCCACGCCCTTTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa          <  2:325474/90‑1 (MQ=255)
                                                          cACGAGCCAGACCCCCCCAGCCCCCACGACCATTTTTCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGa          <  2:255360/90‑1 (MQ=255)
                                                            cGACCCGGACCCCCCCACCCCCCCCGCCCGTTTTCCCGGGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACt        <  1:277913/90‑1 (MQ=255)
                                                                  aGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCGGCAACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtataa  >  1:178537/1‑87 (MQ=255)
                                                                        |                                                                                   
GGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTA  >  NZ_CP009273/3679309‑3679464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑