Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,912	G→T	W30C (TGG→TGT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,912	0	G	T	100.0%	44.2 / NA	13	W30C (TGG→TGT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/7);  total (6/7)

TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAA  >  NZ_CP009273/49827‑49995                                                                                      |                                                                                    tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTa                                                                                 <  2:263063/90‑1 (MQ=255)    gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAAc                                                                              <  1:250121/90‑1 (MQ=255)    gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAAc                                                                              <  1:327540/90‑1 (MQ=255)              ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                    >  1:4226/1‑90 (MQ=255)                   tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCTGTTTTAAACGCAACACCTtaaata                                                               <  2:270795/90‑1 (MQ=255)                                                                  ctgcctacCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                >  1:145357/1‑90 (MQ=255)                                                                   tgcctaccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt               >  1:162392/1‑90 (MQ=255)                                                                       taccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGcc           <  2:145357/88‑1 (MQ=255)                                                                            aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc      <  1:25199/90‑1 (MQ=255)                                                                              ctcGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                               >  1:379335/1‑47 (MQ=255)                                                                              ctcGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                               <  2:379335/47‑1 (MQ=255)                                                                               tcGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCa   >  1:94971/1‑90 (MQ=255)                                                                                cGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa  >  1:146184/1‑90 (MQ=255)                                                                                      |                                                                                    TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAA  >  NZ_CP009273/49827‑49995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑