| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 807,177 | 0 | T | G | 53.8% | 8.4 / 13.2 | 13 | V67G (GTG→GGG) | bioC | malonyl‑ACP O‑methyltransferase BioC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGA > NZ_CP009273/807092‑807261 | ccACAGCGTAAATACACCCACGTACGGGGCGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACg < 1:272009/90‑1 (MQ=255) acCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAAGTCACGCGCAGGGGACGGCCTTAGAtctctcc > 2:217257/1‑89 (MQ=255) acCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTTGAtctctcc > 2:185370/1‑89 (MQ=255) gTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCa < 2:181557/90‑1 (MQ=255) gcgGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCACGCGGCGGGGGCGGCCTTTGATCTCTCGCCGCCCATGCTtttt > 1:265405/1‑90 (MQ=255) gtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCAc < 1:325412/90‑1 (MQ=255) ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGGCGGCCTTAGATCTCTCGCCCCCCATGCTTTTTCAGGCACGCCCGa > 1:45460/1‑90 (MQ=255) ggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAGGATg > 2:214260/1‑90 (MQ=255) gccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg < 1:217257/90‑1 (MQ=255) ccACTGGCGGGAAAGGCACCCGCGGGGGACGGCCCTAGATCCCTTCCCGCCCATGCTTGTTCAGGCACGCCAAAAGGGGGCCGCAGaacc > 2:312338/1‑87 (MQ=255) ggAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGc > 2:136014/1‑90 (MQ=255) tCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGgaga > 1:24991/1‑90 (MQ=255) gcAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGa < 1:185370/90‑1 (MQ=255) | CCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGA > NZ_CP009273/807092‑807261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |