| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,980,476 | 0 | A | C | 53.8% | 12.6 / 14.9 | 13 | Y24S (TAC→TCC) | ftnB | non‑heme ferritin‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCAC > NZ_CP009273/1980387‑1980547 | ggATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa > 1:297730/1‑90 (MQ=255)ggATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa > 1:47272/1‑90 (MQ=255) taAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTc > 2:320438/1‑90 (MQ=255) ggATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCAcc < 2:358042/90‑1 (MQ=255) aTGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg > 1:131253/1‑90 (MQ=255) aTGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg < 1:320438/90‑1 (MQ=255) aTGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg < 2:191984/90‑1 (MQ=255) acctctAAAATGACCCGCGAGTTTTACGCATCCAATCTCTCCCTTCCCCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGcc < 2:297730/88‑1 (MQ=255) cAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTc > 2:100523/1‑90 (MQ=255) acccGCGATTTTTCCGCATCCATTCTCTCCCTCCCCCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCCCCGCCACTTTCCTTcgc < 1:48294/88‑1 (MQ=255) acccGCGAGTTTTACGCATCCAATCTCTCCCTTCCCCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTcgc < 2:335514/88‑1 (MQ=255) gcgATTTTTCCGCCCCCAATCTCCCCCTCCCCCTGAGTAACTGGTTTTCTGACCAGTTTTTAAGCGGCACCGCCACTTTCCTTCGCGccc < 1:140689/90‑1 (MQ=255) tttACGCATCCAATCTCTCCCTTCCCCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGcac < 2:143999/90‑1 (MQ=255) tttACGCATCCAATCTCTCCCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAAGGGCACCGCCACTTTCCTTCGCGCCCAGGcac < 2:333213/90‑1 (MQ=255) tttACGCATCCAATCTCTCCCTCCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGcac < 2:59545/90‑1 (MQ=255) | GGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCAC > NZ_CP009273/1980387‑1980547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |