| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,319,929 | 0 | T | G | 58.8% | 3.5 / 18.5 | 17 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (10/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA > NZ_CP009273/2319849‑2320012 | tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaaa > 2:171742/1‑89 (MQ=255) tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAaaat > 1:133446/1‑90 (MQ=255) ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATg < 1:236257/90‑1 (MQ=255) cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGtt > 2:96106/1‑90 (MQ=255) ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc < 1:96106/90‑1 (MQ=255) tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATTGAAAAAATGAGTTTAGcccc > 2:236257/1‑90 (MQ=255) gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGGTTTGcccccccct > 1:18827/1‑86 (MQ=255) gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTc < 2:287511/90‑1 (MQ=255) cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc < 1:272960/90‑1 (MQ=255) cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc < 1:266396/90‑1 (MQ=255) aggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAGAAGAGATTAAGCCCCCACCTACTCGGTGCAAAAACACCCTCTTGTTAACg > 2:272960/1‑90 (MQ=255) aggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTTGCCCCCCACTTAATCGATGCAAAAGCACGCCCTGGGTAttg > 2:266396/1‑88 (MQ=255) gTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTTGCCCCCCCCTTACTCGATGGCAAAACACGCCCTGGTTATCGTc > 1:20545/1‑90 (MQ=255) gCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAt < 1:294736/90‑1 (MQ=255) gCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAt < 2:134511/90‑1 (MQ=255) ggggggGATGGAAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGGACGGACAGGGTTTTGa > 2:381338/1‑90 (MQ=255) ggggggGATGGAAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGGCAGGGTTAtta > 2:14446/1‑88 (MQ=255) | TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA > NZ_CP009273/2319849‑2320012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |