| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,292,475 | 0 | T | G | 55.0% | 16.6 / 19.2 | 20 | T141P (ACC→CCC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (11/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCAT > NZ_CP009273/4292388‑4292556 | tcaGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGttt < 2:58407/90‑1 (MQ=255) aGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGc < 2:26454/90‑1 (MQ=255) ggATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGGAAATa > 2:321862/1‑90 (MQ=255) gagGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTTCCGTAAAAAGCggc > 2:310502/1‑88 (MQ=255) gagGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTGCCGGAAAAAGCggc > 1:177238/1‑88 (MQ=255) ccacAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTTCCCCTAAAAAGCGACAAAGGGGGCGGCGaaaaa > 2:231593/1‑90 (MQ=255) acaGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGTAAAAAACGACAAAGGCGGCGGCGgaaaaaa > 2:379110/1‑88 (MQ=255) acaGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAAAAACGACAATGGGGACGGCGAAAAAGa > 2:55666/1‑90 (MQ=255) aGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGTTTGCCCGGAAATAACGACAATGGCGGCGGCGGAAAAAAcc > 1:53643/1‑90 (MQ=255) aGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGGCCCGAAAAAGCGACAAAGGGGACGGGGAAAAAGAcc > 2:244100/1‑90 (MQ=255) aGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGAcc < 1:69856/90‑1 (MQ=255) aGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGAcc < 2:278550/90‑1 (MQ=255) gCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAAAAACGACAATGGCGGCGGCGAAAAAAACCa > 2:75516/1‑90 (MQ=255) ttAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGCAAAAAACGACAAAGGGGACGGCGGAAAAAACCAGcc > 1:282040/1‑90 (MQ=255) ttCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGGAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTgcg < 1:168017/90‑1 (MQ=255) gTCGAGCATGGCGGGGGAGGGTTGCCCGTAAAAAACGACAATGGCGACGGCGAAAAAAAACAGCCCCCAACGATGGGGGCGCCTCaaaaa > 2:349569/1‑90 (MQ=255) gTCGAGCATGGCGGGGAAGGGTTGCCCGTAAATAGCGACAATGGGGGCGGCGAAAAAAACCACCCCCAAACGGTGGGGGCGCAGCaaaaa > 2:192254/1‑90 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 1:349569/90‑1 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 1:310502/90‑1 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 1:244100/90‑1 (MQ=255) ggggAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGCCCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCAt < 2:205009/90‑1 (MQ=255) | TCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCAT > NZ_CP009273/4292388‑4292556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |