| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,329,945 | 0 | T | G | 58.8% | 10.7 / 17.1 | 17 | T132P (ACC→CCC) | adiA | arginine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (10/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGC > NZ_CP009273/4329861‑4330031 | gcgcTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATcg > 1:253097/1‑90 (MQ=255) atTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGGCATCGCGGCAACGGcgcggc > 1:65619/1‑88 (MQ=255) atTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGGCATCGCGGCAACGGCGCGTc > 2:126930/1‑90 (MQ=255) atTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATCCCGGCCACGgcgccgc > 1:359466/1‑87 (MQ=255) cATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCgg < 2:55282/90‑1 (MQ=255) tGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGGCATCGCGGCAACGGCGCGGCCGGCg > 1:35573/1‑90 (MQ=255) ttCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTcggcggga > 1:107729/1‑88 (MQ=255) tcatcaGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGGGGGGCATCGCGGCAAAGGGGCGGCCGGCGATAAAAGcggcggggt > 1:3577/1‑87 (MQ=255) atcaGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCt < 1:200443/90‑1 (MQ=255) aGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGGCATCGCGGCAACGGGGCGTCCCGCGCTAAAATCGGCGGGAACTTcc > 2:254376/1‑90 (MQ=255) aGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATCGCGGCAACGGGGCGTCCCGCGATAAAGTCGGCGGGATCTTcc > 1:352257/1‑90 (MQ=255) gTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATCCCGGCAACGGGGCGGCCGGGGAAAAAGGCGGGGGGATCTTCCCGAAACCCggggg > 1:345513/1‑87 (MQ=255) ggcAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCa > 2:99509/1‑90 (MQ=255) ggcAACAGCTGCTGGCGGTAGCGGGGCATCGCGGCAACGGCGCGTCCGGCGATAAAGGCGGCGGGATCTTCCCGAATCCAGGCGAATTCa > 1:132014/1‑90 (MQ=255) gctgctGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACaa < 2:107729/90‑1 (MQ=255) gctgctGGCGGTAGCGAGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACaa < 1:296980/90‑1 (MQ=255) gggTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTcgc < 1:232816/90‑1 (MQ=255) | GCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGC > NZ_CP009273/4329861‑4330031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |