| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 276,800 | 0 | A | C | 52.9% | 14.9 / 19.7 | 17 | Y299D (TAT→GAT) | yagA | IS481‑like element ISEc19 family transposase |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGG > NZ_CP009273/276717‑276877 | gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGa > 1:186028/1‑90 (MQ=255)gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGa > 2:184440/1‑90 (MQ=255)gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGa > 2:234794/1‑90 (MQ=255) tCCCCTTTCCTGACCACCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGccc < 2:209180/90‑1 (MQ=255) tttCCTGACCCCCCCCCCTTCATCGTATCCGGGGGGCGTTGTGTTGCCGTTGTCCTGCCGCCCGGACGGCTGATCCCGCGAGCCGGGtcc < 1:210918/90‑3 (MQ=255) ttcccTGCCCACCCCCCCTTCATCGTTTTCCGGGGGCGTTGTTTTGCCGCTGTACTGCCGCGCTGACGGCTGATCCCGCGAGCCCGGTAc < 1:101699/87‑1 (MQ=255) ttaccaccCCCCCTCCATCGTTTTCCGGGTGCGTTGTTTTCCCCTTCTCCTGCCGCGCGGAAGGCTGATACCGCGAGCCCGGTACCGCCa < 1:26372/88‑1 (MQ=255) cGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTc < 2:39669/90‑1 (MQ=255) gggggCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCa > 2:203079/1‑90 (MQ=255) ggggTGTTTTTTTGCCGGGGTCCGCCCGCTCGGACGGCTGATCCCGCGACCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCaa < 2:207853/90‑1 (MQ=255) ggggCTTTGTTTCCCCGCTGTCCCCCCGCGCGGAGGGCAGATCCCGCGAGCCGGGCCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCaa < 1:184440/90‑1 (MQ=255) ggggCTTTGTGTTCCCGCTGTACGGCCGCGCGGACGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCaa < 2:106498/90‑1 (MQ=255) ggggCGTTGTGTTCCCGCTGTACTGCCGCGCTGCCGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCaa < 2:74255/90‑1 (MQ=255) cGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGtt > 2:8060/1‑90 (MQ=255) tGTCCTGCCGCGCTGACGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGcc < 2:213798/90‑1 (MQ=255) gcTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAg > 2:39292/1‑90 (MQ=255) cTGCCGGCTGATCCCGCGGGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAgg < 2:309414/90‑1 (MQ=255) | GATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGG > NZ_CP009273/276717‑276877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |