Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9930TG53.8% 8.0 / 12.2 13V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAC  >  NZ_CP009273/349907‑350057
                                                                                      |                                                                
aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCggtgggtg                                                                >  1:317969/1‑89 (MQ=255)
aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTg                                                                <  2:317969/89‑1 (MQ=255)
aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTgg                                                               >  1:116779/1‑90 (MQ=255)
aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTgg                                                               >  2:160791/1‑90 (MQ=255)
      cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTggggggga                                                         >  2:40814/1‑86 (MQ=255)
        ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAg                                                       >  2:327842/1‑90 (MQ=255)
                               cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAgggggcg                                >  2:49014/1‑90 (MQ=255)
                                           gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                    <  1:117236/90‑1 (MQ=255)
                                           gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                    <  1:277119/90‑1 (MQ=255)
                                                  tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAGGCGCTGGAAGGGGGGGGGGGGGGGGCGGTTGTCTTTTCGa             >  1:244096/1‑90 (MQ=255)
                                                       aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGGAagag        >  1:51623/1‑90 (MQ=255)
                                                           aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAagagaggg    >  2:88350/1‑88 (MQ=255)
                                                           aTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGGGCTGGAAAGGTGGGTGGGGGGGGGGTTTGTTATTGCCAAaaaggggg    >  2:383010/1‑86 (MQ=255)
                                                             ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGACGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc  <  2:54863/90‑1 (MQ=255)
                                                                                      |                                                                
AATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAC  >  NZ_CP009273/349907‑350057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑