Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273497,0310AC54.5% 7.2 / 11.7 11V555G (GTG→GGG) ybaLKef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCG  >  NZ_CP009273/496968‑497120
                                                               |                                                                                         
gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                                                 >  1:73986/1‑90 (MQ=255)
 tCTTATCATCCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCcagca                                                                <  1:313563/90‑1 (MQ=255)
      tCATCCCTACATTTTTTTCATTTTTTACCCCCGCCACCCCCCTTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc                                                           <  2:282538/90‑1 (MQ=255)
          gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                                       >  2:345734/1‑90 (MQ=255)
           ccTACATTTTTTTAATTTTTTACATCCGGCACCCACCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt                                                      <  1:347776/90‑1 (MQ=255)
                     tttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCg                                            >  1:112259/1‑90 (MQ=255)
                              ttACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                   <  2:95678/90‑1 (MQ=255)
                                                gTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGa                 >  1:145196/1‑90 (MQ=255)
                                                        cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc         >  2:292561/1‑90 (MQ=255)
                                                         gTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacca        <  1:205347/90‑1 (MQ=255)
                                                         gTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacca        <  1:99796/90‑1 (MQ=255)
                                                              caccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc   >  2:308702/1‑90 (MQ=255)
                                                               accTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg  >  1:146579/1‑90 (MQ=255)
                                                               |                                                                                         
GTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCG  >  NZ_CP009273/496968‑497120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑